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Registros recuperados : 19 | |
3. | | KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; SILVA, J. B. G. D.; PEREIRA, L. F. P.; BENASSI, M. T. Green coffee beans composition, roasted coffee and beverages characteristics: diversity between arabica cultivars. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 107 Biblioteca(s): Embrapa Café. |
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7. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Application of near infrared spectroscopy for green coffee biochemical phenotyping. Journal of Near Infrared Spectroscopy, v. 22, n. 6, p. 411-421, 2014. Biblioteca(s): Embrapa Café. |
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9. | | GARRETT, R.; SCHMIDT, E. M.; PEREIRA, L. F. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; EBERLIN, M. N.; REZENDE, C. M. Discrimination of arabica coffee cultivars by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry and chemometrics. LWT - Food Science and Technology, v. 50, n. 2, p. 496-502, 2013. Biblioteca(s): Embrapa Café. |
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10. | | KITZBERGER, C. S. G.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; SERA, T.; SILVA, J. B. G. D.; BENASSI, M. de T. Diterpenes in green and roasted coffee of Coffea arabica cultivars growing in the same edapho-climatic conditions. Journal of Food Composition and Analysis, v. 30, n. 1, P. 52-57, 2013. Biblioteca(s): Embrapa Café. |
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11. | | IVAMOTO-SUZUKI, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; KITZBERGER, C. S. G.; DOMINGUES, D. S.; BOHLMANN, J.; PEREIRA, L. F. P. Functional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L. Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 15863-15873, 2023. Biblioteca(s): Embrapa Café. |
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12. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PAGIATTO, N. F.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Chemical composition in wild ethiopian Arabica coffee accessions. Euphytica, v. 209, n. 2 p. 429-438, 2016. Biblioteca(s): Embrapa Café. |
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13. | | PAGIATTO, N. F.; IVAMOTO, S. T.; SERA, T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D.; PEREIRA, L. F. P. Evaluation of kahweol and cafestol levels in Ethiopian Coffea arabica L. accessions grown in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 217 Biblioteca(s): Embrapa Café. |
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14. | | IVAMOTO, S. T.; CALEDÓN, J. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; BOHLMANN, J.; DOMINGUES, D. S.; PEREIRA, L. F. P. Transcriptome analysis and characterization of genes involved in diterpene biosynthesis in coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 105 Biblioteca(s): Embrapa Café. |
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15. | | IVAMOTO, S. T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; ANDRADE, D. S.; ALVES, L. C.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. In silico analysis of cytochrome p450 genes involved in the metabolism of diterpenes in Coffea. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos... Biblioteca(s): Embrapa Café. |
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16. | | SCHOLZ, M. B. S.; PAGIATTO, N. F.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; CHARMETANTE, P.; LEROYE, T. Validation of near-infrared spectroscopy for the quantification of cafestol and kahweol in green coffee. Food Research International, v. 61, p. 176-182, 2014. Biblioteca(s): Embrapa Café. |
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17. | | IVAMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, MARIA B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Plant Physiology and Biochemistry, v. 111, p. 340-347, Dec. 2016. Biblioteca(s): Embrapa Café. |
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18. | | IVOMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Plant Physiology and Biochemistry, v. 111, p. 340-347, February, 2017. Biblioteca(s): Embrapa Café. |
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19. | | SANT'ANA, G. C.; PEREIRA, L. F. P.; POT, D.; IVAMOTO, S. T.; DOMINGUES, D. S.; FERREIRA, R. V.; PAGIATTO, N. F.; SILVA, B. S. R. da; NOGUEIRA, L. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; OLIVEIRA, F. F. de; SERA, G. H.; PADILHA, L.; LABOUISSE, J-P.; GUYOT, R.; CHARMETANT, P.; LEROY, T. Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L . Scientific Reports, v. 8, n. 465, p. 1-12, 2018 Biblioteca(s): Embrapa Café. |
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Registros recuperados : 19 | |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
13/04/2017 |
Data da última atualização: |
17/04/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
IVAMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, MARIA B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. |
Afiliação: |
SUZANA T. IVAMOTO, IAPAR/UEL; LEONARDO M. SAKURAY, IAPAR/UEL; LUCIA P. FERREIRA, IAPAR; CÍNTIA S. G. KITZBERGER, IAPAR; MARIA B. S. SCHOLZ, IAPAR; DAVID POT, CIRAD; THIERRY LEROY, CIRAD; LUIZ G. E. VIEIRA, UNOESTE; DOUGLAS S. DOMINGUES, UNESP; LUIZ FILIPE PROTASIO PEREIRA, SAPC. |
Título: |
Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 111, p. 340-347, Dec. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Lipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30?240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. MenosLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30?240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warra... Mostrar Tudo |
Palavras-Chave: |
Cafestol; Expressão genética; Kahweol; RT-qPCR. |
Thesagro: |
Biossíntese; Café; Coffea Arábica; Composto químico; Gene; Lipidio. |
Thesaurus NAL: |
Gene expression; High performance liquid chromatography. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/159038/1/Diterpenes-biochemical-profile.pdf
|
Marc: |
LEADER 02756naa a2200373 a 4500 001 2068512 005 2017-04-17 008 2016 bl uuuu u00u1 u #d 100 1 $aIVAMOTO, S. T. 245 $aDiterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development.$h[electronic resource] 260 $c2016 520 $aLipids are among the major chemical compounds present in coffee beans, and they affect the flavor and aroma of the coffee beverage. Coffee oil is rich in kaurene diterpene compounds, mainly cafestol (CAF) and kahweol (KAH), which are related to plant defense mechanisms and to nutraceutical and sensorial beverage characteristics. Despite their importance, the final steps of coffee diterpenes biosynthesis remain unknown. To understand the molecular basis of coffee diterpenes biosynthesis, we report the content dynamics of CAF and KAH in several Coffea arabica tissues and the transcriptional analysis of cytochrome P450 genes (P450). We measured CAF and KAH concentrations in leaves, roots, flower buds, flowers and fruit tissues at seven developmental stages (30?240 days after flowering - DAF) using HPLC. Higher CAF levels were detected in flower buds and flowers when compared to fruits. In contrast, KAH concentration increased along fruit development, peaking at 120 DAF. We did not detect CAF or KAH in leaves, and higher amounts of KAH than CAF were detected in roots. Using P450 candidate genes from a coffee EST database, we performed RT-qPCR transcriptional analysis of leaves, flowers and fruits at three developmental stages (90, 120 and 150 DAF). Three P450 genes (CaCYP76C4, CaCYP82C2 and CaCYP74A1) had transcriptional patterns similar to CAF concentration and two P450 genes (CaCYP71A25 and CaCYP701A3) have transcript accumulation similar to KAH concentration. These data warrant further investigation of these P450s as potential candidate genes involved in the final stages of the CAF and KAH biosynthetic pathways. 650 $aGene expression 650 $aHigh performance liquid chromatography 650 $aBiossíntese 650 $aCafé 650 $aCoffea Arábica 650 $aComposto químico 650 $aGene 650 $aLipidio 653 $aCafestol 653 $aExpressão genética 653 $aKahweol 653 $aRT-qPCR 700 1 $aSAKURAY, L. M. 700 1 $aFERREIRA, L. P. 700 1 $aKITZBERGER, C. S. G. 700 1 $aSCHOLZ, MARIA B. S. 700 1 $aPOT, D. 700 1 $aLEROY, T. 700 1 $aVIEIRA, L. G. E. 700 1 $aDOMINGUES, D. S. 700 1 $aPEREIRA, L. F. P. 773 $tPlant Physiology and Biochemistry$gv. 111, p. 340-347, Dec. 2016.
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